It can be used to measure the potency and stability of ligands or agonist antibodies that can bind and activate OX40. This two-part series begins by walking through the basic considerations for choosing the optimal experimental reporter. Sensitive Quantification of SARS-CoV-2 Viral RNA in Wastewater. Open the BOSSA program. pmirGLO Dual-Luciferase miRNA Target Expression Vector. The Trypsin/Lys-C Mix, Mass Spec Grade, is a mixture of Trypsin Gold, Mass Spectrometry Grade, and rLys-C, Mass Spec Grade. An Introduction to Transfection. Extract High-Quality Nucleic Acids with Less Hands-On Time. In part two, Assay Normalization Options", experimental design and data analysis. The Lumit™ IFN-γ (Human) Immunoassay reagents were added directly to treated cell wells to detect IFN-γ release from the T cells. The Dual-Glo® Luciferase Assay System is a homogeneous reagent system that enables fast and simple quantitation of a stable luminescent signal from two reporter genes in a single sample. Polymer4 is optimized for fragment analysis, and. The PCR Master Mix is designed for routine endpoint PCR for DNA amplicons in the range of 0. Categories Quality Assurance & Regulatory Affairs. So I bought the M3D printer from Kickstarter when they first came out, and just tried it out now. The Lumit™. Your price: Log in. Signal transduction cascades are generally triggered by the binding of ligands, such as growth factors, cytokines, neurotransmitters, or hormones, to a receptor. Abstract. Links to the entire contents of the Promega product catalog are accessible from this page. The NanoBRET™ PPI Starter Systems provide the vectors required to create NanoLuc® Luciferase and HaloTag® protein fusions to target proteins of interest, a Positive Control Pair (p53, MDM2) and the NanoBRET™ Nano-Glo® Detection System, which contains the substrate used by NanoLuc® Luciferase to generate the donor signal and the HaloTag. Please Enquire. Detect SARS-CoV-2 viral RNA in preprocessed wastewater with high efficiency and sensitivity using the SARS-CoV-2 RT-qPCR Kit for Wastewater. In phosphate buffers cleavage occurs at the aspartic and glutamic residues. The final PCR product was inserted into pGEM-T Easy (Promega), generating plasmid pPfRh5_C-term_LoxP. 44μmol) was injected 7- and 13-days post-transduction followed by in vivo imaging. Lumit™ HMGB1 Immunoassay reagents were added directly to the second plate of treated cells after 24 hours of treatment. Because JM109 is rec A– and lacks the E. The Promega mission statement is: To be the most responsive supplier of biological reagents and reagent systems used in research and applied technology applications worldwide. In the absence of IL-2, no signaling occurs downstream of IL-2R and no. The Nano-Glo® Luciferase Assay System provides a simple, single-addition reagent that generates a glow-type signal in the presence of NanoLuc® luciferase with a half-life of approximately 120 minutes in commonly used tissue culture media. CW Spin Baskets. So far it seems to work ok. Trypsin/Lys-C Mix, Mass Spec Grade. 0 Cell Viability Assay exhibits the same high performance as the classic CellTiter-Glo® Assay, making it suitable for use from benchtop scale to high-throughput screening. Instructions for Use of Product (s) C1141, C1145. Product Components and Storage Conditions PRODUCT SIZE CAT. 5ml (100/pack) DNA IQ™ Spin Baskets (50/pack) Promega forensic products are manufactured in alignment with the ISO 18385 standard. ®Maxwell CSC Instrument and Tablet PC The Maxwell® CSC Instrument is controlled by Maxwell® CSC software on a touchscreen Tablet PC that is. In this study the performance of the 2X Rapid Ligation Buffer is compared with that of the previously supplied T4 DNA Ligase 10X Buffer in both one-hour and 16-hour. The Student Resource Center is exactly what you’re looking for. An Extended-Life Dual-Luciferase Assay with "Add-Mix-Measure" Convenience. Catalog number selected: V5285. Generally, 100-fold greater sensitivity can be achieved over. The NanoBiT® PPI Starter Systems provide the vectors required to create LgBiT and SmBiT protein fusions, along with control vectors and the Nano-Glo® Live Cell Assay System, a single-addition, non-lytic detection reagent used for monitoring NanoBiT® luminescence in living cells. US. Categories Quality Assurance & Regulatory Affairs. The GoTaq® qPCR and RT-qPCR Systems are ready-to-use, 2X master mixes containing BRYT Green® Dye, a fluorescent DNA binding dye with minimal PCR inhibition, providing maximum amplification efficiency and greater fluorescence enhancement than SYBR® Green I. $ 14. There are five basic steps of DNA extraction that are consistent across all the possible DNA purification chemistries: 1) disruption of the cellular structure to create a lysate, 2) separation of the soluble DNA from cell debris and other insoluble material, 3) binding the DNA of interest to a purification matrix, 4) washing proteins and other contaminants away from. Lactate dehydrogenase uses lactate and NAD+ to produce pyruvate and NADH. Only T7 DNA or DNA cloned downstream from a T7 promoter can serve as a template for T7 RNA Polymerase-directed RNA synthesis. Optimized conditions enable amplification of as few as 2 copies of target template. NanoLuc® Binary Technology (NanoBiT™) is a two-subunit system based on NanoLuc® luciferase that can be used for intracellular detection of protein:protein interactions (PPIs). Over the past year, Promega has been recognized with additional. The Spectrum Compact CE System is simple to set up and operate, with consumables like buffers and polymer prefilled in convenient packaging. The Alexa Fluor® 488 Ligand is impermeable to cell membranes and, therefore, used to label cell surface proteins. The [A] at which E is 50% of E max is termed the half maximal effective concentration and is abbreviated EC 50, or rarely [A] 50. Key components of a PCR amplification reaction are a thermostable DNA polymerase, Deoxynucleotide. Add to Cart. It is designed for automated extraction of DNA from buccal swabs using the Maxwell® RSC and RSC 48 Instruments. $ 55. Achieve better consistency in detecting low level mixtures. Dec 12, 2023 · On Thursday November 9 th, 2023, Promega held its 7 th Biologics Symposium at the Babraham Research Campus in Cambridge. When engaged with either an anti-TCR/CD3 stimulus alone or an anti-TCR/CD3 and an anti-CD28 stimulus, receptor-mediated signaling induces luminescence. The phMGFP Vector contains the open reading frame for the Monster Green® Fluorescent Protein cloned into a mammalian expression vector. Add ViaFect™ Transfection Reagent and mix by pipetting. $ 73. Some of these cookies are essential for our website to work. Lactate is produced by glycolysis, a major metabolic pathway responsible for glucose homeostasis and energy production. There are five basic steps of DNA extraction that are consistent across all the possible DNA purification chemistries: 1) disruption of the cellular structure to create a lysate, 2) separation of the soluble DNA from cell debris and other insoluble material, 3) binding the DNA of interest to a purification matrix, 4) washing proteins and other contaminants away from. Promega's Cookie Policy We use cookies and similar technologies to make our website work, run analytics, improve our website, and show you personalized content and advertising. Finally this plasmid was linearized with EcoRI, mixed. This page includes product listings and examples of how functional bioassays are used throughout biologics drug development workflows. GoScript uses M-MLV reverse transcriptase and state-of-the-art buffer to drive robust, reliable cDNA synthesis of a full range of rare and. Working at Promega means growing and developing with a very innovative company that stands for progress and knows how to combine economic and social aspects. The 5X GoTaq® Green and Colorless Reaction Buffers supplied with GoTaq® DNA Polymerase contain MgCl 2 at a concentration of 7. Instructions for Use of Product(s) J1631. The master mix allows sensitive detection and quantification even in the presence of reverse transcriptase inhibitors or PCR inhibitors. Promega products and technologies are used. 1,000 assays. In this study the performance of the 2X Rapid Ligation Buffer is compared with that of the previously supplied T4 DNA Ligase 10X Buffer in both one-hour and 16-hour ligation. Explore more information on the Spectrum Compact CE System, as well as data and video resources. Caroline End, Product Manager, Promega Germany. RT-PCR kits and standalone reverse transcriptase enzymes for reverse transcription of full-length cDNA from your experimental sample. Promega Corporation. The Quantus™ Fluorometer is optimized with preprogrammed settings for Promega QuantiFluor® Dye Systems for nucleic acid quantitation and allows the flexibility to create. Promega Corporation. Literature # TM491. LDH-Glo™ Cytotoxicity Assay Technical Manual. NanoLuc® Binary Technology (NanoBiT™) is a two-subunit system based on NanoLuc® luciferase that can be used for intracellular detection of protein:protein interactions (PPIs). Optimized conditions enable amplification of as few as 2 copies of target template. See below for details of the vectors provided with each system. Each topic is unique and has proven its popularity by our Promega Connections readers! Take a look at our top three most-viewed blog posts of 2023. Req ID: 3686. We invite you to discover a career that means more by bringing your full self every day and taking on the challenges that. ELISA (Enzyme Linked Immunosorbent Assays) and Western blot techniques are typical examples of commonly used immunoassays. A protocol for the pGL4. Add to Cart. The OX40 Bioassay is a bioluminescent cell-based assay that overcomes the limitations of existing assays. Add to Cart. Literature # TM318. Renilla luciferase is used as the primary reporter gene, and the gene of interest. Promega forensic products are manufactured in alignment with the ISO 18385 standard. A9281, A9282, A9285. Tel: (86) 10 5825 6268 Fax: (86) 10 5825 6160 Toll-Free Phone: 400 810 8133 E-mail Address: info@promega. Restriction Enzyme Tables. The Student Resource Center is exactly what you’re looking for. Reporter bioassays and cell health assays are used to characterize and develop novel monoclonal antibody (mAb)-based therapeutics, cell and gene therapies, vaccines, and RNA therapeutics. The assay consists of a genetically engineered Jurkat T cell line that expresses human. Restriction Enzyme Tables. The OX40 Bioassay is a bioluminescent cell-based assay that overcomes the limitations of existing assays. The NanoBRET™ PPI Starter Systems provide the vectors required to create NanoLuc® Luciferase and HaloTag® protein fusions to target proteins of interest, a Positive Control Pair (p53, MDM2) and the NanoBRET™ Nano-Glo® Detection System, which contains the substrate used by NanoLuc® Luciferase to generate the donor signal and the HaloTag. PCR Master Mix allows you to set up your reactions in less than a minute—just add template and primers. In PCR, a DNA molecule is targeted and copied multiple times, allowing exponential amplification of the target DNA sequence. Optimized conditions enable amplification of as few as 2 copies of target template. High sensitivity and broad linearity. GoTaq® G2 is supplied in a proprietary formulation containing 50% glycerol with buffers designed for enhanced amplification. Working at Promega means growing and developing with a very innovative company that stands for progress and knows how to combine economic and social aspects. This standard ensures minimal risk of human DNA contamination for products used to collect, store and analyze biological materials for forensic purposes. The subunits are fused to proteins of interest, forming a functional enzyme that generates a bright, luminescent signal when the proteins interact. Thus a protein with a mass of 64kDa has a molecular weight of 64,000 grams per mole. Lactate dehydrogenase (LDH) is a soluble stable cytosolic enzyme present in many cell types that is rapidly released into the cell culture medium upon disruption of the plasma membrane. Each topic is unique and has proven its popularity by our Promega Connections readers! Take a look at our top three most-viewed blog posts of 2023. Our product portfolio of over 4,000 products supports a range of life science work in cell biology, DNA, RNA and protein analysis, drug development, human identification and molecular diagnostics. Literature # TM318. Mitoxantrone produces a dose- and time-dependent release of extracellular ATP (left) and a dose-dependent release of HMGB1 (right) with potency agreement between the two measurements of damage-associated molecular. Catalog number selected: V5071. Our product portfolio of over 4,000 products supports a range of life science work in cell biology, DNA, RNA and protein analysis, drug development, human identification and molecular diagnostics. Transfection is the process of introducing nucleic acids into eukaryotic cells by nonviral methods. Promega Australia will be closed over the holidays from 5:00 pm Thursday, 21 December 2023 until 8:30 am Monday, 8 January 2024. The Rapid-Digestion Trypsin and Rapid-Digestion Trypsin/Lys-C Kits are designed to shorten protein digestion times to 60 minutes versus the typical 4–18 hours required for trypsin digestion. Restriction Enzyme Tables. Your price: Log in. Detect SARS-CoV-2 viral RNA in preprocessed wastewater with high efficiency and sensitivity using the SARS-CoV-2 RT-qPCR Kit for Wastewater. 0 Assay is proportional to the number of viable Jurkat cells in. In PCR, a DNA molecule is targeted and copied multiple times, allowing exponential amplification of the target DNA sequence. HaloTag® TMR Ligand, 15ul. The DNA is suitable for Southern blot hybridizations, genomic analysis (including PCR) and genomic library. The Lactate-Glo™ Assay is a bioluminescent assay for rapid, selective and sensitive detection of L-Lactate in biological samples. The master mix allows sensitive detection and quantification even in the presence of reverse transcriptase inhibitors or PCR inhibitors. 5mM in the 1X. Dilute DNA by adding OptiMEM™ media and DNA to a microfuge tube. PCR Basics. Applied Biosystems ABI PRISM® 7000 and 7700 Sequence Detection System; Applied Biosystems 7300 and 7900HT Real-Time PCR System; Applied Biosystems GeneAmp® 5700 Thermal Cycler;. Revenue is approaching $450 million (USD) in 2019. JM109 Competent Cells are available for convenient transformation in two efficiencies: High Efficiency at greater than 10 8. Contains the cellular components necessary for protein synthesis (tRNA, ribosomes, initiation, elongation and termination factors). The "no cell" blank control value has been subtracted from each. The capillary array is positioned behind the oven door and lies flat against the oven, ensuring consistent capillary temperatures during electrophoresis. Or you’re just trying to get a job. Promega offers an extensive toolbox of reporter bioassays to characterize and develop novel monoclonal antibody (mAb) and cell-based therapeutics. The cDNA prepared from each reaction using this system may. Luciferin-IPA/10ml Assay. 69,561 followers. The new GoTaq® qPCR Master Mix is a 2X master mix for quantitative PCR (qPCR) and features GoTaq® Hot Start Polymerase, a full-length Taq DNA polymerase bound to a proprietary antibody that prevents polymerase activity at room temperature. The low elution volume is used to generate concentrated high-quality RNA suitable for use in downstream applications such as quantitative RT-PCR. The development of reporter gene systems and selection. At Promega Corporation, creativity and connection drive discovery and innovation. Mitoxantrone produces a dose- and time-dependent release of extracellular ATP (left) and a dose-dependent release of HMGB1 (right) with potency agreement between the two measurements of damage-associated molecular. HaloTag® Fluorescent Ligands enable you to add the chloroalkane group, with which HaloTag® protein reacts, to any compound or surface with a compatible chemical group. Promega's Cookie Policy We use cookies and similar technologies to make our website work, run analytics, improve our website, and show you personalized content and advertising. Native trypsin is subject to autolysis, generating pseudotrypsin. Dive into a Research Highlight looking into combining organotypic culture models with high throughput screening (HTS) assays as a rapid, cost-efficient. Mitoxantrone produces a dose- and time-dependent release of extracellular ATP (left) and a dose-dependent release of HMGB1 (right) with potency agreement between the two measurements of damage-associated molecular patterns. The Promega Applications Guide is a comprehensive PDF document that provides useful information on various topics related to molecular biology, such as DNA and RNA purification, PCR, cloning, gene expression, cell culture, and more. Two warranty levels allow you to customize your support solution. The Biomath Calculators provide a range of functions essential to molecular biology experiments, including nucleic acid and protein conversions, melting temperature, temperature conversion, molarity and dilution calculations. In the presence of NADH a pro-luciferin. The enzyme will not join single-stranded nucleic acids. The master mix is composed of an optimized buffer formulation complete with dNTPs and. The subunits are fused to proteins of interest, forming a functional enzyme that generates a bright, luminescent signal when the proteins interact. 7 ± 0. In the absence of an antibody analyte. The kit contains the same trusted reagents as the DNA IQ™ System in a. OX40 Bioassay is a bioluminescent cell-based assay that measures the potency and stability of ligands or agonist antibodies that can bind and activate OX40. PCR, the polymerase chain reaction, is a core technique that has revolutionized molecular biology. DNA-PK is thought to play a role in controlling gene regulation and cell growth. Promega Corporation is a worldwide leader in applying biochemistry and molecular biology to the development of innovative, high-value products for the life sciences. The specialist will also explain all of the sections of the instrument log book. Maxwell provides consistent purification and eliminates concerns about clogged tips or contaminating drips. The HiBiT Control Protein is a 20μM solution of purified recombinant 36kDa HaloTag® protein fused at its carboxy terminus to the 11-amino-acid HiBiT tag. Promega product catalog supplement 2021–2022. Use with both Maxwell® FSC DNA IQ™ Casework Kit and DNA IQ™ Casework Pro Kit for Maxwell® 16. The Spectrum Compact CE System is an integrated instrument for sequencing and fragment analysis. Note: Adjust total volume of diluted DNA for the appropriate number of wells being transfected. Complete listing of products for academic research, drug discovery, clinical research and diagnostics, applied sciences and human. To a microfuge tube or a V-bottom plate, a dd 10µl of diluted DNA. [2] The company now offers more than 4,000 life science products used by scientists, researchers and life science and pharmaceutical companies. We offer a full range of luciferase assay products and tools for your research. The AMV Reverse Transcriptase synthesizes single-stranded cDNA from total or poly (A)+ RNA. Your price: Log in. Instructions for Use of Product (s) C1141, C1145. Over the past year, Promega has been recognized with additional. A guide to cell-free protein expression systems. The master mix is provided as a ready-to-use, stabilized 2X formulation that includes all components for qPCR (except template, primers and probe). The Lumit™ FcRn Binding Immunoassay is a competition assay to measure the interaction between human FcRn and Fc proteins, including antibodies. Literature # TM548. Your price: Log in. The Maxwell® RSC Instrument is a compact, automated nucleic acid purification platform that processes up to 16 samples simultaneously. Contains the cellular components necessary for protein synthesis (tRNA, ribosomes, initiation, elongation and termination factors). Purified CD8+ T cells (effector cells) were combined with Raji B cells (target cells) and a serial dilution of Blincyto® (CD3 and CD19 bispecific T cell engager). Purity and yield of the extracted nucleic acids is key to performance in downstream applications such as PCR and sequencing. Promega Corporation was founded by Bill Linton in 1978 to provide restriction enzymes for biotechnology. Promega Corporation is a worldwide leader in applying biochemistry and molecular biology to the development of innovative, high-value products for the life sciences. 0 Cell Viability Assay exhibits the same high performance as the classic CellTiter-Glo® Assay, making it suitable for use from benchtop scale to high-throughput screening. Let's find the product that meets your needs. Promega Corporation is a amazon app download for android, gayforitwu
A protocol for the pGL4. . Proemga
$ 59. Add to Cart. 50µl Conductive Disposable Tips, Filtered/60/box (5,760 tips) Consumable products such as tips, plates, reagent reservoirs and waste bags that are used with the Maxprep™ Liquid Handler. GoTaq® DNA Polymerase is a proprietary formulation of Taq polymerase that gives robust amplification equal to and, in some cases, superior to that of standard Taq. Polymer4 is optimized for fragment analysis, and. Luminescence measured with the CellTiter-Glo® 2. The low elution volume is used to generate concentrated high-quality RNA suitable for use in downstream applications such as quantitative RT-PCR. The Maxwell® RSC Instrument is a compact, automated nucleic acid purification platform that processes up to 16 samples simultaneously. Products include preprocessing reagents for swabs and card punches prior to direct amplification of database samples, and DNA isolation systems for casework samples. The Lumit™ IFN-γ (Human) Immunoassay reagents were added directly to treated cell wells to detect IFN-γ release from the T cells. Promega (Beijing) Biotech Co. The Dual-Glo® Luciferase Assay System is a homogeneous reagent system that enables fast and simple quantitation of a stable luminescent signal from two reporter genes in a single sample. The one thing I’m not recollecting 100% is whether there were different versions of the app based on the bundled firmware. JM109 Competent Cells. [2] The company now offers more than 4,000 life science products used by scientists, researchers and life science and pharmaceutical companies. Your price: Log in. The data show that digestion at low pH was comparable to digestion at conventional conditions. 6kDa) and Small BiT (SmBiT; 11 amino acids) subunits are fused to proteins of interest, and when expressed, the PPI brings the subunits into. Promega Corporation is a worldwide leader in applying biochemistry and molecular biology to the development of innovative, high-value products for the life sciences. The Dual-Glo® Luciferase Assay System is a homogeneous reagent system that enables fast and simple quantitation of a stable luminescent signal from two reporter genes in a single sample. Reverse transcriptases (RTs) are RNA-directed DNA polymerases that synthesize a DNA copy (cDNA) from a target RNA. Promega offers an extensive toolbox of reporter bioassays to characterize and develop novel monoclonal antibody (mAb) and cell-based therapeutics. Here we demonstrate how luciferase technology can be a powerful tool in your research program across a wide area of focus. Optimized conditions enable amplification of as few as 2 copies of target template. This master mix does not contain a reference dye; however, a. The Lactate-Glo™ Assay is a bioluminescent assay for rapid, selective and sensitive detection of L-lactate in biological samples. Maxwell® Instruments offer benchtop automation with worry-free, walk-away purification of DNA, RNA or total nucleic acid. Use with both Maxwell® FSC DNA IQ™ Casework Kit and DNA IQ™ Casework Pro Kit for Maxwell® 16. Extraction of DNA, RNA or total nucleic acids from samples such as blood, cultured cells, microbes or plant and animal tissues is critical to the success of many experiments. Their abundance has been estimated at 10 7 −10 9 virus-like particles (VLPs)/ml in sediments, oceans, and soil [1, 2], and 10 9 −10 12 VLPs/g feces in the human gut []. Zatrudnia ponad 1400 pracowników i oferuje ponad 4000 produktów z dziedziny biologii molekularnej, analizy ekspresji i interakcji białek, analizy komórek, poszukiwania nowych leków, diagnostyki molekularnej jak i kryminalistyki oraz badań pokrewieństwa. The IL-2 Bioassay consists of a genetically engineered cell line, IL-2 Bioassay Cells. WBE has previously been used to detect the presence of pharmaceutical or industrial. The assays are designed to measure the activities of P450s from recombinant and native sources and for testing the effects of analytes such as drugs and new chemical entities on P450 activities. The P450-Glo™ CYP3A4 Assays provide a homogeneous, luminescent method for measuring cytochrome P450 CYP3A4 activity. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 2 www. An Extended-Life Dual-Luciferase Assay with "Add-Mix-Measure" Convenience. 6kDa) and Small BiT (SmBiT; 11 amino acids) subunits are fused to proteins of interest, and when expressed, the PPI brings the subunits into. The low elution volume is used to generate concentrated high-quality RNA suitable for use in downstream applications such as quantitative RT-PCR. Single-step molarity and dilution calculations. The 1,000bp and 3,000bp fragments have increased intensity relative to the other bands on ethidium bromide. The HiBiT Control Protein can be used as a positive control of known concentration when using the Nano-Glo® HiBiT Lytic Detection System (Cat. Promega Corporation careers and job. This award recognizes Promega for not only offering great benefits, but for putting employees first, unlocking their potential and inspiring their growth and performance. Promega has 1,601 employees. $ 151. This method takes advantage of the “A” overhang added by a PCR enzyme like Taq DNA Polymerase. G8252 PDF (200 KB) – English (United States). The Lactate-Glo™ Assay is a bioluminescent assay for rapid, selective and sensitive detection of L-Lactate in biological samples. The DNA fragments may be stained with ethidium bromide. 5ml (100/pack) DNA IQ™ Spin Baskets (50/pack) Promega forensic products are manufactured in alignment with the ISO 18385 standard. NanoLuc® Binary Technology (NanoBiT™) is a two-subunit system based on NanoLuc® luciferase that can be used for intracellular detection of protein:protein interactions (PPIs). Total RNA was isolated from leaf samples collected five days post-infection using the Promega MaxWell® RSC Plant RNA Kit (AS1500), as per the manufacturer's instructions using a Promega MaxWell RSC robotic system. HaloTag® TMR Ligand, 15ul. Please refer to the product label for the intended use statements for specific. Contains the cellular components necessary for protein synthesis (tRNA, ribosomes, initiation, elongation and termination factors). Molecular Weight (g/mol) Final Concentration. 24 [luc2P/minP] Vector, which encodes the luciferase reporter gene luc2P and is designed for high expression and reduced anomalous transcription. JM109 is a K strain that is rec A– and end A– to minimize recombination and improve the quality of plasmid DNA. Maxwell® Instruments offer benchtop automation with worry-free, walk-away purification of DNA, RNA or total nucleic acid. Genetic reporters are used as indicators to. pansy), represent a large part of the Promega luminescence portfolio. Nuclease-Free Water is an essential component of molecular biology experiments. GoTaq® qPCR Master Mix is provided as a simple-to-use, stabilized 2X master mix that includes all components for quantitative PCR except sample DNA, primers and water. Links to the entire contents of the Promega product catalog are accessible from this page. Because JM109 is rec A– and lacks the E. This award recognizes Promega for not only offering great benefits, but for putting employees first, unlocking their potential and inspiring their growth and performance. The Lumit™ IFN-γ (Human) Immunoassay reagents were added directly to treated cell wells to detect IFN-γ release from the T cells. In the assay, a human IgG1 labeled with LgBiT (Tracer-LgBiT) is used as the tracer. RT-PCR kits and standalone reverse transcriptase enzymes for reverse transcription of full-length cDNA from your experimental sample. Elution Tubes, 0. Catalog number selected: V5071. JM109 is a K strain that is rec A– and end A– to minimize recombination and improve the quality of plasmid DNA. The Caspase-Glo® 3/7 Reagent is optimized for caspase activity, luciferase activity and cell lysis. 4 Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 TM040 · Revised 8/23 www. The PCR Master Mix is designed for routine endpoint PCR for DNA amplicons in the range of 0. Once considered merely a byproduct of glycolysis, lactate is now considered an important. Note: Adjust total volume of diluted DNA for the appropriate number of wells being transfected. PCR Nucleotide Mix is manufactured under cGMP conditions and has equimolar amounts of each dNTP to ensure optimal PCR. Our product portfolio of over 4,000 products supports a range. Lumit™ HMGB1 Immunoassay reagents were added directly to the second plate of treated cells after 24 hours of treatment. Upon purchase of the Spectrum Compact CE System, the instrument will be covered by a one-year Standard Warranty. The Lumit™. $ 97. Generally, 100-fold greater sensitivity can be achieved over. com PRODUCT USE LIMITATIONS, WARRANTY, DISCLAIMER Promega manufactures products for a number of intended uses. Learn how Promega can. The vector enables monitoring of changes in expression of a target gene fused to a reporter gene. Your price: Log in. Basic Isolation Procedure. Trypsin is a serine protease that specifically cleaves at the carboxylic side of lysine and arginine residues. com PRODUCT USE LIMITATIONS, WARRANTY, DISCLAIMER Promega manufactures products for a number of intended uses. Complementary DNA (cDNA) was synthesized using 1 µg of total RNA, an oligo(dT)20 primer and the Superscript III reverse. The OX40 Bioassay, Propagation Model is a bioluminescent cell-based assay that overcomes the limitations of existing assays. Instructions for Use of Product(s) J1631. A simple, one-step quantitative assay for the detection of biologically active interleukin-2 (IL-2) is described. The strain grows well and is transformed efficiently by a variety of methods. Promega was named a top employer in the US for a second year in a row, winning a 2023 Top Workplaces USA award. 0 Assay is proportional to the number of viable Jurkat cells in. The CellTiter 96® AQ ueous One Solution Reagent contains a tetrazolium compound [3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4. Convenient Luminescence Signal Monitors RNAi Effect. A guide to cell-free protein expression systems. Promega Corporation was founded by Bill Linton in 1978 to provide restriction enzymes for biotechnology. Promega was named a top employer in the US for a second year in a row, winning a 2023 Top Workplaces USA award. Linear results are seen over at least eight orders of magnitude of enzyme concentration, and less than 10 –20 moles of luciferase can be measured under optimal conditions. Position Type Regular Full-Time. Includes an overview of the methods and systems available for in vitro translation of proteins, including eukaryotic and prokaryotic-based cell free expression systems. . craigslist dallas jobs